The efficient microscale synthesis of \(\left[1-{ }^{14} \mathrm{C}\right]\) propionyl-CoA from commercially available sodium \(\left[1-{ }^{14} \mathrm{C}\right]-\) propionate using 1,1 'carbonyldiimidazole in yields of nearly \(\mathbf{7 0 \%}\) is reported for the first time. A substantial improv
Synthesis of radiolabeled acetyl-coenzyme A from sodium acetate
β Scribed by Richard C. Clough; Susan R. Barnum; Jan G. Jaworski
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 283 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## An enzymatic synthesis of ["Cl acetyl coenzyme A from [l-l'c] acetate is described. "COZ was reacted with methylmagnesium bromide in diethyl ether at 0 'C. The excess of Grignard reagent was hydrolyzed by adding methanol. After evaporation of the solvents, the residue was redissolved in hydroch
## Abstract Sodium acetateβ^18^O~2~ is synthesized by the hydrolysis of triethyl orthoacetate with waterβ^18^O in 89% chemical yield with a 1.1 to 1.2βfold dilution of the isotopic label contained in waterβ^18^O.
Procedures for the preparation of UDP-N-[1-14C]acetyl-D-glucosamine and UDP-N-[1-14C]acetyl-D-galactosamine with very high specific activities are described. The overall yield based on the amount of [1-14C]acetate used is greater than 80%. The N-acetyl-D-glucosamine-alpha-1-phosphate used in this sy