## Abstract Rat forebrain astrocytes synthesize heat shock proteins with molecular weights 97,89,70,68, and 30β34 kilodaltons. The stress inducible 68βkDa heat shock protein (HSPβ68) was vigorously expressed by astroβcytes in culture after a 45Β°C, 20 min heat shock. HSPβ68 synthesis was poorly indu
Synthesis of heat shock proteins in rat liver after ischemia and hyperthermia
β Scribed by Gaetano Cairo; Lidia Bardella; Luisa Schiaffonati; Aldo Bernelli-Zazzera
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 609 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0270-9139
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β¦ Synopsis
Ischemia of rat liver is followed by recovery or cell death. Since heat shock proteins may be essential to cell survival under stress, we determined levels of heat shock proteins in liver after different periods of blood deprivation and correlated the results with cellular recovery. Cell-free synthesis by poly (A+)-mRNA and polysomes revealed 70 and 89 kd proteins which appear similar to proteins produced by the liver of rats with amphetamine-induced hyperthermia. The 70 and 89 kd proteins increased in the liver of rats which recovered from ischemia.
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Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody