𝔖 Bobbio Scriptorium
✦   LIBER   ✦

THE LEVEL AND PHOSPHORYLATION OF HSP70 IN THE RAT LIVER CYTOSOL AFTER ADRENALECTOMY AND HYPERTHERMIA

✍ Scribed by Aleksandra Čvoro; Jadranka Dundjerski; Divna Trajković; Gordana Matić

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
400 KB
Volume
23
Category
Article
ISSN
1065-6995

No coin nor oath required. For personal study only.

✦ Synopsis

Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing both constitutive and inducible forms of the protein.

A comparison between basal and heat stress-induced levels of the protein in the liver cytosol of the three groups of animals suggested that glucocorticoid hormones stimulate the basal synthesis of Hsp70 and inhibit its induction by stress. In both unstressed and hyperthermia-exposed animals, hepatic Hsp70 was detected as a phosphoprotein. The extent of its in vitro phosphorylation was found to be significantly reduced by heat stress or adrenalectomy, but dexamethasone failed to restore it to the original level.

📜 SIMILAR VOLUMES

Effects of hyperthermia on xanthine oxid
Effects of hyperthermia on xanthine oxidase activity and glutathione levels in the perfused rat liver
✍ Skibba, Joseph L. ;Stadnicka, Anna ;Kalbfleisch, John H. ;Powers, Robert H. 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 645 KB

The hepatotoxic effects of hyperthermic liver perfusion were investigated in male Fischer 344 rat livers. Perfusions were carried out at 37, 41, 42, 42.5, and 43°C for 2 hr. During the 2 hr, the perfusate was analyzed for activity of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), N-a

Methoxychlor-induced alteration in the l
Methoxychlor-induced alteration in the levels of HSP70 and clusterin is accompanied with oxidative stress in adult rat testis
✍ S. Vaithinathan; B. Saradha; P. P. Mathur 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB

## Abstract Methoxychlor, an organochlorine pesticide, has been reported to induce abnormalities in male reproductive tract. However, the insight into the mechanisms of gonadal toxicity induced by methoxychlor is not well known. We investigated whether treatment with methoxychlor would alter the le

Expression of heat shock genes (hsp70) i
Expression of heat shock genes (hsp70) in the mammalian brain: Distinguishing constitutively expressed and hyperthermia-inducible mRNA species
✍ I. R. Brown; S. J. Rush 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 605 KB

The mammalian genome contains both constitutively expressed and heat-shock-inducible members of the hsp70 gene family. Riboprobes derived from members of these two classes of heat shock genes were utilized in Northern blot studies to analyze brain mRNA isolated from control rabbits and rats and from

Enhancement of neutral phosphatase activ
Enhancement of neutral phosphatase activity in the cytosol and nuclei of regenerating rat liver: Role of endogenous regucalcin
✍ Masami Omura; Masayoshi Yamaguchi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 121 KB 👁 2 views

The role of endogenous regucalcin (RC) in the regulation of neutral phosphatase activity in regenerating rat liver was investigated. The liver weight reduced by a partial hepatectomy (about 70%) was completely restored at 72 h after surgery. Phosphotyrosine, phosphoserine, and phosphothreonine were

Tumor necrosis factor and endotoxin in t
Tumor necrosis factor and endotoxin in the pathogenesis of liver and pulmonary injuries after orthotopic liver transplantation in the rat
✍ Moritaka Goto; Dr. Yoshiyuki Takei; Sunao Kawano; Shingo Tsuji; Hiroyuki Fukui; 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 797 KB

This study examines whether tumor necrosis factor and endotoxin are involved in the pathogenesis of primary nonfunction of graft and pulmonary complication after orthotopic liver transplantation. Livers from Lewis rats were stored for either 1 or 4 hr in ice-cold Em-Collins solution (1-hr storage an