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Synthesis of DNA-binding protein in WI-38 cells stimulated to synthesize DNA by medium replacement

✍ Scribed by B.-K. Choe; N.R. Rose

Book ID
118876445
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
836 KB
Volume
83
Category
Article
ISSN
0014-4827

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## Abstract When resting WI‐38 cells in a confluent monolayer were stimulated to proliferate by changing the medium, the incorporation of leucine‐^3^H into nuclear acidic proteins was promptly stimulated, although its incorporation into total cellular proteins was unchanged or even decreased. Three

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Diploid human fibroblasts in culture (WI 38) were allowed to reach a stationary phase and were then stimulated to reenter DNA synthesis and cell division by addition of serum to the culture medium. The rate of protein synthesis increased during the first hours after addition of serum reaching at thr