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Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum

✍ Scribed by F. Wiebel; R. Baserga

Publisher: John Wiley and Sons
Year: 1969
Tongue: English
Weight: 889 KB
Volume: 74
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040740211

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✦ Synopsis

Diploid human fibroblasts in culture (WI 38) were allowed to reach a stationary phase and were then stimulated to reenter DNA synthesis and cell division by addition of serum to the culture medium. The rate of protein synthesis increased during the first hours after addition of serum reaching at three hours a plateau value that continued for at least 24 hours after serum addition. Inhibition of protein synthesis during the early hours after serum addition abolished the stimulation of DNA synthesis occurring 20 to 28 hours later.

Increased protein synthesis was preceded by a rapid decrease in the intracellular pool size of most amino acids. These changes were independent of concomitant protein synthesis. They suggest that serum exerts an immediate effect on the function of the cell membrane.

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Early changes in the synthesis of acidic

Early changes in the synthesis of acidic nuclear proteins in human diploid fibroblasts stimulated to synthesize DNA by changing the medium

✍ Giovanni Rovera; Renato Baserga 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 English ⚖ 886 KB

## Abstract When resting WI‐38 cells in a confluent monolayer were stimulated to proliferate by changing the medium, the incorporation of leucine‐^3^H into nuclear acidic proteins was promptly stimulated, although its incorporation into total cellular proteins was unchanged or even decreased. Three