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Hydrocortisone stimulation of DNA synthesis in bromodeoxyuridine-selected non-dividing WI-38 cells

โœ Scribed by P.D. Phillips; K. Woolwich; V.J. Cristofalo

Book ID
119025353
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
381 KB
Volume
20
Category
Article
ISSN
0047-6374

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โœ Scott D. Gorman; Vincent J. Cristofalo ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 620 KB

Bromodeoxyuridine-selected nondividing senescent WI-38 cells were stimulated to synthesize DNA, as evidenced by incorporation of [3H]thymidine into nuclei of senescent cells, after infection with simian virus 40 (SV40). Cellular DNA synthesis was confirmed by DNA-DNA hybridization experiments and th

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## Abstract In quiescent confluent monolayers of WIโ€38 cells, the specific activity of the tRNA methyltransferases falls to 20% of the level found in log phase cells. When the resting cells are stimulated to proliferate by a change to fresh medium, the enzymes show a rapid rise in specific activity