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Synthesis of [D-Ala2, D-Leu5]enkephalin tritiated in the 2,6-positions of tyrosine

✍ Scribed by Hiroshi Hasegawa; Yoshihiko Shinohara; Shigeo Baba

Publisher: John Wiley and Sons
Year: 1996
Tongue: French
Weight: 351 KB
Volume: 38
Category: Article
ISSN: 0022-2135
DOI: 10.1002/(sici)1099-1344(199609)38:9<825::aid-jlcr907>3.0.co;2-a

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✦ Synopsis

D-Ma2, D-Leu51Enkephalin (DADLE) labelled with tritium in the 2,6-positions of N-terminus tyrosine residue has been prepared. Synthesis of the precursor peptide, [2,6-dibromo-Tyrl]DADLE, was performed by solid phase synthesis using F'moc strategy.

The peptide was tritiated catalytically to yield [2,6-3H-Tyrl] DADLE with a specific radioactivity of 1.61 TBq/mmol. The distribution of the tritium was investigated by HPLC with radioisotope detection following hydrolysis with aminopeptidase M, and confirmed that the label was entirely located at the tyrosine residue.

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