Synthesis of 14C-ochratoxin A

✍ Scribed by D. Rousseau; G. Slegers; C. Van Peteghem; A. Claeys

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: French
• Weight: 344 KB
• Volume: 21
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580210506

✦ Synopsis

14C-0chratoxin A s obtained by coupling t h e N-hydroxysuccinimide d e r i v a t i v e of t h e l a c t o n e a c i d h y d r o l y s a t e o f n a t u r a l o c h r a t o x i n A w t h 14C-L-8-phenylalanine methylester. 14C-Ochratoxin A inethylester i s converted i n t o 14Gochratox i n A by a l k a l i n e h y d r o l y s i s . P u r i f i c a t i o n o f t h e intermedia t e s and t h e 1 4 C -o c h r a t o x i n A i s achieved by HPLC. For an amount o f 0.05 pmole L-6-phenylalanine methylester t h e y i e l d o f ochratoxin A i s 0.02 pmole o r 40 % and t h e s p e c i f i c a c t iv i t y 130 Ci/mole. S t e r e o s p e c i f i c i t y i s 100 % and radiochemic a l p u r i t y is 96 96.

The y i e l d o f 1%-ochratoxin A increases w i t h increasing amounts o f 14C-L-pphenylalanine methylester and reaches 80% under t h e best conditions.