Ochratoxin A labelled with 14C or 3H in the phenylalanine moiety

✍ Scribed by Karl Hult

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: French
• Weight: 200 KB
• Volume: 23
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580230802

✦ Synopsis

Ochratoxin A is a mycotoxin produced by Asperqillus and Penici1l.w moulds. The toxin is an amide formed between phenylalanine and the isocoumarin acid ochratoxin a .

Ochratoxin A with high specific radioactivity can be obtained, by sub titution of the natural phenylalanine for I4C or %-labelled phenylalanine.

Labelled ochratoxin A was formed by condensation of labelled phenylalanine ethylester with an anhydride derivative of ochratoxin a. The intermediate 0-acetyl ochratoxin C was hydrolysed with pig liver esterase and chymotrypsin to afford ochratoxin A. The overall yield of radioactivity was 50%. Specifi activi ies up to 500 Ci/mol and obtained.