Synthesis in vitro of very long chain fatty acids inVibriosp. strain ABE-1

✍ Scribed by Naoki Morita; Nobuhiro Okajima; Masaru Gotoh; Hideyuki Hayashi; Hidetoshi Okuyama; Shoji Sasaki

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 656 KB
• Volume: 157
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00245154

✦ Synopsis

The activity of fatty acid synthetase (FAS) from Vibrio sp. strain ABE-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type II FAS. These observations indicate that this enzyme is a type II FAS. Analysis by gas-liquid chromatography of the reaction products synthesized in vitro from [2-14C]malonyl-CoA by the partially purified FAS revealed, in addition to 16- and 18-carbon fatty acids which are normal constituents of this bacterium, the presence of fatty acids with very long chains. These fatty acids were identified as saturated and mono-unsaturated fatty acids with 20 up to as many as 30 carbon atoms. The longest fatty acids normally found in this bacterium contain 18-carbon atoms. These results suggest that the FAS from Vibrio sp. strain ABE-1 has potentially the ability to synthesize fatty acids with very long chains.