Synthesis, biological activity and solution structure of new analogues of the antimicrobial Gramicidin S

✍ Scribed by Elżbieta Kamysz; Beata Mickiewicz; Wojciech Kamysz; Sylwia Bielińska; Sylwia Rodziewicz-Motowidło; Jerzy Ciarkowski

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 295 KB
• Volume: 17
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/psc.1314

✦ Synopsis

Abstract

Gramicidin S (GS) is a cyclo‐decapeptide antibiotic isolated from Bacillus brevis. The structural studies have shown that GS forms a two‐stranded antiparallel β‐sheet imposed by two II′ β‐turns. Despite its wide Gram+ and Gram− antimicrobial spectrum, GS is useless in therapy because of its high hemotoxicity in humans. It was found, however, that the analogues of GS‐14 (GS with 14 amino acid residues) attained a better antimicrobial selectivity when their amphipatic moments were perturbed. In this study, we report effects of similar perturbations imposed on GS cyclo‐decapeptide analogues. Having solved their structures by NMR/molecular dynamics and having tested their activities/selectivities, we have concluded that the idea of perturbation of the amphipatic moment does not work for GS‐10_0 analogues. An innovative approach to the synthesis of head‐to‐tail cyclopeptides was used. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.