Synthesis and micellar formation of spin-labeled lysolecithin

✍ Scribed by Hideo Utsumi; Keizo Inoue; Shoshichi Nojima; Takao Kwan

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 465 KB
• Volume: 19
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(77)90043-3

✦ Synopsis

A spin-labeled lysolecithin, 1-[ 12'-(N-oxyi-4",4"-dimethyloxazolidine)-stearoyl] -snglycero-3-phosphorylcholine, has been synthesized in which the spin is covalently attached to its fatty acyl chain.

The electron spin resonance spectra of this lysolecithin in an aqueous solution generally showed sharp three resonance lines superposed on a broad resonance line.

Investigation of changes in the signal intensity of these spectra against the concentration of lysolecithin led to the inference that the sharp lines are due to monomers of lysolecithin while the broad one to micelles. The critical micellar concentration was consistent with that evaluated from the spectral shift of a dye.

In the electron spin resonance spectra obtained from spin-labeled lysolecilhin solutions with various amounts of dimyristoyllecithin, the line width of broad signal ansed from micellar spin-labeled iysolecithin broadened as increase of dimyristoyllecithin. The linebroadening thus observed was briefly discussed.