✦ LIBER ✦

Synthesis and Crosslinking of L-DOPA Containing Polypeptide Vesicles

✍ Scribed by Eric P. Holowka; Timothy J. Deming

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 455 KB
Volume: 10
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.200900390

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The synthesis and self‐assembly of DOPA containing diblock copolypeptides into spherical vesicles is described. DOPA residues are naturally abundant in mussel adhesive proteins and are responsible for extensive covalent crosslinking of these materials upon oxidation. We found that vesicles could be formed from copolypeptides containing different amounts of DOPA substituted into hydrophobic segments, up to 100% DOPA content. The DOPA containing vesicles were covalently crosslinked in water using an oxidizing agent, in a process similar to the crosslinking of mussel adhesive proteins, which gave vesicles with dramatically improved membrane stability against freeze‐drying, organic solvent, osmotic stress and complex media. These materials showed greatly enhanced membrane stability compared to non‐crosslinked vesicles and have the advantage that the biomimetic crosslinker DOPA can be incorporated directly into the polypeptide sequence during synthesis.

magnified image

📜 SIMILAR VOLUMES

DOPA-containing polypeptides. I. Improve

DOPA-containing polypeptides. I. Improved synthesis of high-molecular-weight poly(L-DOPA) and water-soluble copolypeptides

✍ W. D. Fuller; M. S. Verlander; M. Goodman 📂 Article 📅 1978 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 272 KB 👁 1 views

## DOPA-Containing Polypeptides. I. Improved Synthesis o f High-Molecular-Weight Poly( L-DOPA) and Water-Soluble Copolypep tides Despite considerable interest in the pharmacological properties of L-DOPA (3,4-dihydroxy-1,-phenylalanine), the synthesis of polypeptides containing the optically active

Synthesis of Sequential Polypeptides Con

Synthesis of Sequential Polypeptides Containing O-Phospho-L-Serine

✍ Hiroyuki Yamamoto; Arihiro Saitoh; Kousaku Ohkawa 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

## Abstract The chemistry of phosphoserine [Ser(P)] containing peptides and polypeptides was extensively investigated to explore a new biomineralization material science. The selective cleavage of the __O__,__O′__‐diphenyl phospho‐protecting groups of Ser(PO~3~Ph~2~) was examined using hydrogenolys

Microbiological synthesis of L-dopa

✍ John Rosazza; Paul Foss; Michael Lemberger; Charles J. Sih 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 493 KB

small, it could not be isolated for further spectral analysis. Therefore, the positions of hydroxy and methoxy substitution on the aromatic system were assigned, based on the following information: (a) the presence of the 163 and 164 pair in the mass spectrum precluded the possibility of any substit

Synthesis and enzymatic decarboxylation

Synthesis and enzymatic decarboxylation of 6-bromo-L-dopa, a potential brain tracer for L-dopa

✍ Michael Wong; Onofre T. de Jesus 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 French ⚖ 288 KB 👁 1 views

Direct brominatbn of L-dopa with stoichbmetric amounts of molecular bromine was found to yield 6-bromo-L-dopa as the sole product. This determination was made using liquid chromatography with electrochemical detector, mass spectroscopy, and proton NMR. 6-Bromo-L-dopa was found to be a substrate of a

Synthesis of N-[11C]-methyl-L-DOPA

✍ Dr. Andrew Horti; Hayden T. Ravert; Robert F. Dannals; Henry N. Wagner Jr. 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 French ⚖ 409 KB

## Abstract The radiochemical synthesis of N‐[^11^C‐methyl]‐L‐DOPA was accomplished by N‐methylation of the methyl and ethyl esters of L‐N‐tert‐butyl‐oxycarbonyl‐[β‐(3,4‐dimethoxyphenyl)] alaninate with [^11^C]iodomethane using sodium hydride in tetrahydrofuran and deprotection of the N‐methyl inte

Synthesis and reactions of self-crosslin

Synthesis and reactions of self-crosslinkable epoxy- and anhydride-containing macromolecules

✍ Zakir M O Rzaev; Ali Güner 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 151 KB 👁 2 views

Synthesis of ternary copolymers of some unsaturated electron-donor epoxide monomers (UEM) such as 4-methyl-4,5-epoxypentene-1 (MEP), vinyl-(VGE), allyl-(AGE) and p-isopropenylphenyl (PhGE) glycidyl ethers, maleic anhydride (MA) and styrene (St) was carried out in methylethyl ketone (MEK) in the pres