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Syntheses and Characterization of Conjugates of Nucleic Acid Probes and 6-Aminoglucose-based Polymers

✍ Scribed by Thierry Delair; Bérengère Badey; Alain Domard; Christian Pichot; Bernard Mandrand

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
356 KB
Volume
8
Category
Article
ISSN
1042-7147

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✦ Synopsis

Homopolymers based on 6-deoxy-6-methacryloylamido-Dglucopyranose

(6-aminoglucose), obtained by radical-initiated polymerization, were used as reactive polymeric supports to tether oligodeoxyribonucleotides (ODNs) in order to make oligonucleotide-polymer conjugates of potential applications in diagnostics. The reducing ends of the homopolymers, used as self-protected groups during the radical polymerizations, served as reactive groups for the covalent attachment of 5Ј-aminomodified oligodeoxyribonucleotides via reductive amination, with sodium borohydride as a reducing agent. The best coupling conditions used dimethylformamide/ 20 mM sodium tetraborate as a 90/10 (v/v) mixture at 50°C. The main factors controlling the course of the reaction are electrostatic repulsive interactions and polymer chain complexation. A low ionic strength was essential for the reaction to occur in a pH range of 7.3 to 9.3. The polymer-ODN conjugates could be aggregated depending on the extent of polymer chain interactions.

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