Conjugation of nucleic acid probes to 6-aminoglucose-based homo- and copolymers. II. Application to diagnostics

✍ Scribed by Thierry Delair; Bérengère Badey; Marie-Hélène Charles; Ali Laayoun; Alain Domard; Christian Pichot; Bernard Mandrand

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 405 KB
• Volume: 8
• Category: Article
• ISSN: 1042-7147
• DOI: 10.1002/(sici)1099-1581(199709)8:9<545::aid-pat685>3.0.co;2-s

✦ Synopsis

The covalent immobilization of oligodeoxynucleotides to a 6-deoxy-6-methacryloylamido-D-glucopyranose (6-amino glucose) homopolymer and a 36% hydroxyethylmethacrylate (HEMA) copolymer was studied in order to make oligodeoxynucleotide-polymer conjugates of potential applications in diagnostics. The reducing ends of the saccharidic moieties of the (co)polymers served as reactive groups for the covalent attachment of 5Ј-amino-modified oligodeoxyribonucleotides via reductive amination. The course of the immobilization reaction was slightly affected by the polymer composition, but differences were marked as far as the aggregation of the conjugates was concerned.

Evaluation of the conjugates in the quantification of hepatitis B virus DNA allowed amplification factors identical to the reference polymer. Amplification was the most efficient with conjugates obtained, at low pH, with the copolymer.