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Covalent immobilization of nucleic acid probes onto reactive synthetic polymers

✍ Scribed by N. Ferraton; Th. Delair; A. Laayoun; P. Cros; B. Mandrand

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 170 KB
Volume: 66
Category: Article
ISSN: 0021-8995
DOI: 10.1002/(sici)1097-4628(19971010)66:2<233::aid-app4>3.0.co;2-x

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✦ Synopsis

Oligodeoxyribonucleotide (ODN)/polymer conjugates were obtained by covalent immobilization of various nucleic acid sequences to three different polymers. Two of them bore active ester groups for the covalent linkage via the formation of amide bonds and the third one featured aldehyde moieties for immobilization via reductive amination. The factors controlling the conjugation reaction were found to be the reactivities of the polymers and their abilities at forming hydrogen bonds with the ODNs to be tethered. It was found, as well, that the observed aggregation of the grafting reaction products was essentially due to hydrogen bonding with the nucleic bases of the tethered oligonucleotides.

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Maleic anhydride-co-methyl vinyl ether copolymers were used as reactive polymers to link oligodeoxyribonucleotides (ODN) to make oligonucleotide-copolymer conjugates of potential applications in diagnostics. The anhydride moieties of the copolymers were used for the covalent binding, via the formati