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Synchronous replication of the parental chromosomes in a Chinese hamster-mouse somatic hybrid

✍ Scribed by G. Marin; P. Manduca

Book ID
118876114
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
311 KB
Volume
75
Category
Article
ISSN
0014-4827

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Chromosome replication in somatic hybrid
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## Abstract Hybrid clones were obtained between a mouse cell line (3TP) and a temperature‐sensitive Chinese hamster cell line (K12) unable to grow at 40Β° because of a __ts__ defect apparently located at the G1/S transition. The great majority of hybrid clones grew at 40Β°, showing the __ts__ defect

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## Abstract Somatic hybrids obtained by the selective method of Littlefield between a permanent line of Chinese hamster cells (Wg3) and one of mouse cells (3TP) showed a preponderance of biarmed (hamster) chromosomes. Under normal culture conditions (37Β°) the doubling time of the parental mouse cel