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Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA

✍ Scribed by Alan H. Rosenberg; Martha N. Simon; F.William Studier; Richard J. Roberts


Book ID
118918157
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
764 KB
Volume
135
Category
Article
ISSN
0022-2836

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Restriction of bacteriophage T3 and T7 o
✍ KrΓΌger, Detlev H. ;Reuter, Monika ;Schroeder, Cornelia ;Glatman, Larisa I. ;Cher πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 444 KB

When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy