𝔖 Bobbio Scriptorium
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Mapping of the XhoI and SacI restriction sites on the bacteriophage T5 DNA

✍ Scribed by Davison, John


Book ID
122939731
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
115 KB
Volume
16
Category
Article
ISSN
0378-1119

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T4 dC-DNA was digested with the restriction endonucleases BglII, SalI and XhoI. Overlaps in the three sets of fragments allowed the mapping of all restriction sites relative to each other along the T4 genome.

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The cytosine-containing DNA of a mutant of bacteriophage T4 was digested with restriction endonucleases SmaI, KpnI and BglII producing 5, 7 and 13 fragments respectively. Complete physical maps of the T4 genome were constructed with the enzymes SmaI and KpnI and an almost complete map with the enzym