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Restriction of bacteriophage T3 and T7 ocr + strains by the type II restriction endonuclease EcoRV

✍ Scribed by Krüger, Detlev H. ;Reuter, Monika ;Schroeder, Cornelia ;Glatman, Larisa I. ;Chernin, Leonid S.

Book ID
104725227
Publisher
Springer
Year
1983
Tongue
English
Weight
444 KB
Volume
190
Category
Article
ISSN
0026-8925

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✦ Synopsis

When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzyme cuts T3 DNA at 5 sites. T7 and its ocr- mutants have no EcoRV sites in their DNA. In contrast to the anti-restriction activity of the T3 and T7 ocr+ gene function against type I and III restriction enzymes, the ocr+ protein is unable to inactivate the type II restriction endonuclease EcoRV.

📜 SIMILAR VOLUMES

Different restriction of bacteriophages
Different restriction of bacteriophages T3 and T7 by Pl-lysogenic cells and the role of the T3-coded SAMase
✍ Dr. D. H. Krüger; W. Presber; Sigrid Hansen; H. A. Rosenthal 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 744 KB

## Abstract The intracellular growth of the phages T3 and T7 is restricted in the presence of the __Escherichia coli__ prophage P1. Phage T3 has a higher ability to express its genome and to damage the host cell than T7. This partial protection of T3 against P1 restriction is due to the T3‐coded SA