𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Different restriction of bacteriophages T3 and T7 by Pl-lysogenic cells and the role of the T3-coded SAMase

✍ Scribed by Dr. D. H. Krüger; W. Presber; Sigrid Hansen; H. A. Rosenthal

Book ID
102909823
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
744 KB
Volume
17
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The intracellular growth of the phages T3 and T7 is restricted in the presence of the Escherichia coli prophage P1. Phage T3 has a higher ability to express its genome and to damage the host cell than T7. This partial protection of T3 against P1 restriction is due to the T3‐coded SAMase, an enzyme which degrades S‐adenosylmethionine, the cofactor of the P1 restriction endonuclease. Since we did not observe DNA cleavage in vivo, we conclude that the in vivo action of the P1 nuclease is limited to a SAM‐dependent repressor‐like binding to T3 and T7 DNA, while further reactions with the DNA (modification vs cleavage) are blocked.

📜 SIMILAR VOLUMES

Restriction of bacteriophage T3 and T7 o
Restriction of bacteriophage T3 and T7 ocr + strains by the type II restriction endonuclease EcoRV
✍ Krüger, Detlev H. ;Reuter, Monika ;Schroeder, Cornelia ;Glatman, Larisa I. ;Cher 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 444 KB

When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy

The binding and processing of plasminoge
The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells
✍ Jerry Tobler; Monty Krieger; Robert M. Stroud 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 989 KB

## Abstract The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells was studied using ^125^I‐labeled canine plasminogen. Throughout a 3‐day period, ^125^I‐plasminogen in the incubation medium bound to the cells and was degraded, first to intermediate‐sized macromolecules that were t