Even illumination in total internal refl
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R. Fiolka; Y. Belyaev; H. Ewers; A. Stemmer
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Article
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2007
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John Wiley and Sons
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English
β 318 KB
## Abstract In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epiβillumination modes. In wideβfield imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform il