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A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy

✍ Scribed by Eisuke Adachi; Yutaka Kazoe; Yohei Sato; Yuko Suzuki; Tetsumei Urano; Takehiko Ueyama; Naoaki Saito; Viacheslav O. Nikolaev; Martin J. Lohse; Makoto Tominaga; Hideo Mogami


Publisher
Springer
Year
2009
Tongue
English
Weight
433 KB
Volume
459
Category
Article
ISSN
0031-6768

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## Abstract A caspase sensor based on Förster resonance energy transfer between fluorescent proteins is reported. Enhanced cyan fluorescent protein anchored to the inner leaflet of the plasma membrane of living cells is optically excited by an evanescent electromagnetic field and transfers its exci