Surface-Enhanced Raman Scattering Based Nonfluorescent Probe for Multiplex DNA Detection
β Scribed by Sun, Lan; Yu, Chenxu; Irudayaraj, Joseph
- Book ID
- 111650583
- Publisher
- American Chemical Society
- Year
- 2007
- Tongue
- English
- Weight
- 378 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0003-2700
No coin nor oath required. For personal study only.
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## Abstract Silverβnanoparticle dimers held together by a Raman reporter, capped with DNA aptamers and stabilized by polyethylene glycol chains, can be used to develop a multiplexed heterogeneous bioassay for protein detection with high sensitivity and selectivity.
The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the