𝔖 Bobbio Scriptorium
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Detection and identification of labeled DNA by surface enhanced resonance Raman scattering

✍ Scribed by D. Graham; B. J. Mallinder; W. E. Smith

Publisher
Wiley (John Wiley & Sons)
Year
2000
Tongue
English
Weight
198 KB
Volume
57
Category
Article
ISSN
0006-3525

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✦ Synopsis

The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 Ο« 10 Οͺ12 M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.

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The resonance Raman scattering (RRS) of 1-(2-pyridylazo)-2-naphthol in the neutral, basic and acidic solutions was reinvestigated. On the basis of the states of polarization of the Raman bands in neutral solution and possible tautomeric forms of the compound in basic, acidic and neutral media, the o