✦ LIBER ✦

Suppression of metastable interference in matrix-assisted laser desorption/ionization (MALDI) spectra in a reflecting time-of-flight mass spectrometer

✍ Scribed by Werner Ens; Victor Spicer; Kenneth G. Standing; Jie Zhou

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 469 KB
Volume: 28
Category: Article
ISSN: 1076-5174
DOI: 10.1002/oms.1210281212

No coin nor oath required. For personal study only.

✦ Synopsis

Metastable interference in matrix

-assisted laser desorption/ionization (MALDI) spectra is reduced by modulating the accelerating potential (by < -100 V) on alternate laser shots. Both parent and daughter ions are reflected on every second shot but only daughter ions are reflected on the next shot. The parent-ion spectrum is then obtained by subtraction. In order to ensure the necessary well defined energy distributions, signals with similar intensity (preferably small) are selected by transferring each transient to a computer for examination. This procedure decreases the complexity in reflected spectra (especially spectra of mixtures) in the lower mass range and improves the mass resolution in the intermediate mass range around 10 000 u. Continuous background in reflected spectra is also suppressed by the technique.

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