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A miniaturized matrix-assisted laser desorption/ionization time of flight mass spectrometer with mass-correlated acceleration focusing

✍ Scribed by Robert D. English; Robert J. Cotter

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
238 KB
Volume
38
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Mass correlated acceleration (MCA) has now been integrated into a 4 in (10.2 cm) matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometer to achieve high resolving power across a broader mass range, without sacrificing detection of higher mass ions. The goal was to combine MCA with a custom‐built miniaturized instrument such as those that might be used for field‐portable applications. Unlike other pulsed extraction methods, MCA is not mass dependent and mass spectra can be achieved with a single tuning of instrument parameters. Additionally, the multi‐channel recording advantage is better realized because ions of all masses can be brought into focus simultaneously. The MCA dual‐stage ion source compensates dynamically for the mass dependence by incorporating an extraction pulse region followed by an acceleration region that contains a time‐dependent waveform correlated with mass. The technique was validated with applications in peptide mixtures and protein digestions. Diagnostic studies for the instrument include m/z range and limits of detection. Copyright © 2003 John Wiley & Sons, Ltd.

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