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Suppression of CD44 expression decreases migration and invasion of human glioma cells

✍ Scribed by Hideho Okada; Jun Yoshida; Masahiro Sokabe; Takashi Wakabayashi; Masatoshi Hagiwara

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
753 KB
Volume
66
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

We have reported high expression of CD44H in human glioma cells. To investigate the role of CD44H in the invasion of human glioma, we established a CD44-anti-sense-gene-expression glioma cell line named U-251AI. The expression of CD44H in the G-4 18-selected U-25 I A l cells was reduced to 2096 of that in the parental U-25ISP cells, as determined by flow-cytometry analysis. We first examined the migratory responses of U-25 I A l cells in vitro by time-lapse video-microscopic sparse cellmigration assay on hyaluronic acid or on chonddtin 6 sulfate. U-251AI cells did not show significant differences in motility on any substrate. while U-25 I SP and other CD44H-positive glioma cells showed dose-de ndent increase of migration specificalty on hyaluronic acid. l feo examine the physiologic function of CD44H in gliomas in vivo, U-25IAI and its control cells, U-25 I S I , which retain CD44-sense-expression vector, werp injected stereotactically into the brains of nude mice. U-25IAI cells were localised in the region of the injection site, with relatively well demarcated borders between tumour and brain tissue, while the control cells demonstrated a cell-infiltration pattern. Our data suggest that C W H may be required for infiltration of glioma cells through its interaction with hyaluronic acid, a major component of the brain extracellular matrix.

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