Hydroxyl radicals may be formed in a xanthine oxidase/hypoxanthine system, where the superoxide anion radical O-.2 and H2O2 are produced. The superoxide-dependent production of the OH. radicals may be monitored by determining the amount of hydroxylated aromatic compounds formed in such a system. Liq
Superoxide-dependent formation of hydroxyl radicals: Detection of hydroxyl radicals by the hydroxylation of aromatic compounds
โ Scribed by Ramsay Richmond; Barry Halliwell; Jasbir Chauhan; Andre Darbre
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 588 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
Hydroxylation of l-phenylalanine (Phe) by hydroxyl radical (\*OH) yields 4-, 3-, and 2-hydroxyl-Phe (para-, meta-, and ortho-tyrosine, respectively). Phe derivative measurements have been employed to detect \*OH formation in cells and tissues, however, the specificity of this assay is limited since
An aromatic substrate for hydroxylation by OH radicals has been synthesized. Neither the substrate nor its hydroxylated product binds either iron(III) or iron(II). A spectrophotometric assay based on the hydroxylation of this substrate, N,N'-(5-nitro-1,3-phenylene)bisglutaramide, is described.
## Abstract The antioxidant behavior of a series of new synthesized substituted thiazolylโthiazolidineโ2,4โdione compounds (TZDs) was examined using chemiluminescence and electron paramagnetic resonance spin trapping techniques. 5,5โDimethylโ1โpyrrolineโ__N__โoxide (DMPO) was used as the spin trap.