Determination of hydroxylated aromatic compounds produced via superoxide-dependent formation of hydroxyl radicals by liquid chromatography/electrochemistry

✍ Scribed by Donna M. Radzik; Daryl A. Roston; Peter T. Kissinger

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 471 KB
• Volume: 131
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90199-9

✦ Synopsis

Hydroxyl radicals may be formed in a xanthine oxidase/hypoxanthine system, where the superoxide anion radical O-.2 and H2O2 are produced. The superoxide-dependent production of the OH. radicals may be monitored by determining the amount of hydroxylated aromatic compounds formed in such a system. Liquid chromatography/electrochemistry is a powerful tool for the determination of hydroxylated aromatic compounds. A technique is presented in which aniline and phenol are hydroxylated in xanthine oxidase/hypoxanthine incubations. No sample derivatization is needed for the determinations which can be accomplished by direct injection of the incubation mixture. Detection limits for 1,2- and 1,4-hydroxylated compounds are in the picomole range.