Super-Resolution Microscopy for Material Science

✍ Scribed by Lorenzo Albertazzi (editor), Peter Zijlstra (editor)

• Publisher: CRC Press
• Year: 2024
• Tongue: English
• Leaves: 249
• Edition: 1
• Category: Library

✦ Synopsis

Optical microscopy is one of the most frequently used tools in chemistry and the life sciences. However, its limited resolution hampers the use of optical imaging to many other relevant problems in different disciplines. Super-Resolution Microscopy (SRM) is a new technique that allows the resolution of objects down to a few billionth of meters (nanometers), ten times better than classical microscopes, opening up opportunities to use this tool in new fields.

This book describes the theory, principles, and practice of super-resolution microscopy in the field of materials science and nanotechnology. There is a growing interest in the applications of SRM beyond biology as new synthetic materials, such as nanoscale sensors and catalysts, nanostructured materials, functional polymers, and nanoparticles, have nanoscopic features that are challenging to visualize with traditional imaging methods.

SRM has the potential to be used to image and understand these cutting-edge man-made objects and guide the design of materials for novel applications.

This book is an ideal guide for researchers in the fields of microscopy and materials science and chemistry as well as graduate students studying physics, materials science, biomedical engineering, and chemistry.

Key Features:

• Contains practical guidance on Super-Resolution Microscopy (SRM), an exciting and growing tool that was awarded the Nobel Prize for chemistry in 2014
• Provides a new perspective targeting materials science, unlike existing books which target readers in chemistry, life science, and biology
• Targets students in its core chapters, while offering more advanced material for professionals and researchers in later chapters

✦ Table of Contents

Cover
Half Title
Title Page
Copyright Page
Table of Contents
Editors
Contributors
Chapter 1: Introduction to Super-Resolution Microscopy and Its Importance for Materials Science
1.1 Introduction
1.2 Fluorescence and Fluorescence Microscopy
1.2.1 Fluorescence and Key Fluorophore Features
1.2.2 Fluorescent Labels
1.2.3 Fluorescence Microscope Layout
1.3 Breaking the Diffraction Limit: Super-Resolution Microscopy
1.3.1 The Diffraction Limit
1.3.2 Breaking the Diffraction Limit: An Historical Perspective on Super-Resolution Microscopy
1.3.3 Families of Super-Resolution Microscopy: A Guide through the Jungle of Acronyms
1.4 Why Super-Resolution Microscopy for Materials?
References
Chapter 2: Localization Microscopy
2.1 Introduction
2.2 Principles of 2D Localization Microscopy
2.3 Localizing the Emitter by Fitting the Point Spread Function (PSF)
2.3.1 Shape of the PSF
2.3.2 Fitting the PSF
2.3.3 Localization Precision and Accuracy
2.3.4 Image Reconstruction
2.4 Methods for Switching Dyes
2.4.1 Photoactivation (PALM)
2.4.2 Photoswitching (STORM)
2.4.3 Switching by Reversible Interactions
2.5 Quantification Approaches
2.6 Application to Materials
2.6.1 Applications to Nanoparticle Imaging
2.6.2 Applications to Polymer Imaging
References
Chapter 3: Stimulated Emission Depletion Microscopy
3.1 Introduction
3.2 Principles of STED
3.2.1 Stimulated Emission Depletion
3.2.2 Optical Setup
3.2.3 Optical Resolution
3.2.4 3D Imaging
3.2.5 Other State Transitions
3.3 Novel Variations on the STED Principle
3.3.1 MINFIELD and DyMIN
3.3.2 MINFLUX
3.4 Application Examples in Materials Science
References
Chapter 4: Structured Illumination Microscopy (SIM)
4.1 Introduction
4.2 SIM Principle
4.2.1 Practical Implementation
4.2.2 SIM Variations
4.3 Applications
4.4 Conclusions
References
Chapter 5: Other Super-Resolution Approaches
5.1 Introduction
5.2 Fluctuation-Based Imaging
5.3 Image Scanning Microscopy
5.4 Near-Field Imaging
References
Chapter 6: Quantitative Analysis for Single-Molecule Localization Microscopy: “From PSF to Information”
6.1 Fitting the Point Spread Function
6.1.1 Model for the PSF
6.1.1.1 The Airy PSF
6.1.1.2 The Vectorial PSF
6.1.2 Fitting
6.1.2.1 Least-Squares
6.1.2.2 Maximum-Likelihood Estimation
6.2 PSF Engineering – Making the PSF More Informative
6.2.1 3D Localization
6.2.1.1 Imaging Multiple Planes
6.2.1.2 Phase Manipulation (PSF Engineering)
6.2.2 Fluorophore Orientation
6.2.2.1 Defocus
6.2.2.2 Back Focal Plane Imaging
6.2.2.3 Polarization
6.2.2.4 Phase Manipulation (PSF Engineering)
6.3 Modulation Enhanced Localization Microscopy
6.3.1 MINFLUX
6.3.2 Modulation Enhanced Localization Microscopy
6.4 Outlook
References
Chapter 7: Single Molecule Localization and Nanoscopy Through Sequential Structured Illumination
7.1 MINFLUX
7.1.1 A Common Framework
7.2 RASTMIN
7.2.1 Outlook
References
Chapter 8: Measuring Molecule Numbers in Nano-Scale Assemblies With Single-Molecule Localization Microscopy
8.1 Introduction
8.2 Extracting Molecule Numbers from Single-Molecule PALM Data
8.2.1 Photophysics of Fluorescent Proteins
8.2.2 Mathematical Models to Describe Blinking Statistics
8.3 Extracting Molecule Numbers from Single-Molecule dSTORM Data
8.4 Discussion
References
Chapter 9: Super-Resolution Microscopy in Colloid Science
9.1 Introduction
9.2 Colloids at Interfaces
9.3 Microgels
References
Chapter 10: SRM Application to Supramolecular Structures
10.1 Overview
10.2 Single-Component Supramolecular Assemblies
10.2.1 Supramolecular Assemblies from Small Molecules
10.2.2 Supramolecular Assemblies from Peptides and Proteins
10.3 Self-Sorted Multi-Component Systems
10.4 Supramolecular Block Assemblies
10.5 Conclusion and Future Perspective
References
Chapter 11: Super-Resolution Microscopy Application to Nanomedicine
11.1 Introduction to Nanomedicine
11.2 Challenges in Nanomedicine
11.3 How Super Resolution Can Help Nanomedicine
11.3.1 Nanomaterials In Vitro Characterization by SRM
11.3.2 SRM to Study Nano-Bio Interactions
11.4 Future Perspectives
11.5 Conclusions
Acknowledgements
References
Chapter 12: Super-Resolution Microscopy Applications to Catalysis
12.1 Introduction
12.2 Layered Double Hydroxides
12.3 Metal Nanoparticles
12.4 Zeolites
12.5 Semiconductor Nanoparticles
12.6 Conclusion and Outlook
References
Index