Two models of sedimentation in a density gradient are analyzed. The firht is f(ir sedimentation in cylindrical sector geometry and contains the assumption that diff i w i o i i can be neglected. The second treats sedimentation in a rectangular field and includediffusion, although the boundaries are
Sucrose density gradient sedimentation of E. coli ribosomes
β Scribed by J.B. Chaires; G. Kegeles
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 605 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0301-4622
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Investigations have been made on the optical rotatory dispersion properties of E. wli ribosomes and their constituent RNA and proteins. The results indicate that (1) no conformational changes are involved in the formation of a 705 particle from the 50s and 30s subunits, ( 2 ) the E. coli ribosomal p