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Optical rotatory dispersion of E. coli ribosomes and their constituents

โœ Scribed by P. K. Sarkar; J. T. Yang; Paul Doty


Publisher
Wiley (John Wiley & Sons)
Year
1967
Tongue
English
Weight
246 KB
Volume
5
Category
Article
ISSN
0006-3525

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โœฆ Synopsis


Investigations have been made on the optical rotatory dispersion properties of E. wli ribosomes and their constituent RNA and proteins. The results indicate that (1) no conformational changes are involved in the formation of a 705 particle from the 50s and 30s subunits, ( 2 ) the E. coli ribosomal proteins are similar to most globular proteins with little a-helix content, and ( 3 ) the conformation of RNA and proteins inside the ribosome is very similar to that in the free state.


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An analysis of optical rotatory dispersion and circular dichroisin of RNA is described which leads to an estimate of the degree of base pairing. By the use of new standards for the double-helical parts of the molecule, based on data for two-stranded viral RNA species, a good fit between calculated a