๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Successful use of the same slide for consecutive fluorescence in situ hybridization experiments

โœ Scribed by Judith Dierlamm; Iwona Wlodarska; Lucienne Michaux; Roberta La Starza; Wolfgang Zeller; Cristina Mecucci; Herman Van den Berghe

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
361 KB
Volume
16
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

โœฆ Synopsis

The feasibility of using the same slide repeatedly for fluorescence in situ hybridization (FISH) experiments was systematically evaluated by applying standard procedures and various combinations of direct-and indirect-labeled probes to slides from patients with hematologic malignancies. Specific and distinct hybridization signals along with weak background signals and chromosome morphology of good to moderate quality could be obtained in up to three experiments performed consecutively on the same slide. Signals related to biotin-or digoxigenin-labeled probes applied in previous hybridizations were still visible with variable intensity, but interpretation problems that may result from this signal noise can be avoided by using adequate probes, detection systems and fluorochromes, and sequence of experiments.

๐Ÿ“œ SIMILAR VOLUMES

Reutilization of previously hybridized s
Reutilization of previously hybridized slides for fluorescence in situ hybridization
โœ Lucy Epstein; Sandy DeVries; Frederic M. Waldman ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 343 KB

Application of fluorescence in situ hybridization (FISH) to clinical material is sometimes limited by sample size. In addition, heterogeneity among slides prepared from a single sample may lead to variation in FISH analyses. Reutilization of material for repeated FISH analyses would help to alleviat

Use of fluorescence in situ hybridizatio
Use of fluorescence in situ hybridization for retrospective detection of aneuploidy in multiple myeloma
โœ Wonbae Lee; Kyungja Han; Rosa M. Drut; Charles P. Harris; Lorraine F. Meisner ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 523 KB

In malignancies with a low mitotic index such as multiple myeloma (MM), conventional cytogenetic studies may not be informative. This study's purpose was to assess specific numerical chromosomal aberrations in non-dividing MM cells by fluorescence in situ hybridization (FISH) of D N A chromosome pro

Interphase fluorescence in situ hybridiz
Interphase fluorescence in situ hybridization assay for the detection of 3q21 rearrangements in myeloid malignancies
โœ Rotraud Wieser; Ulrike Schreiner; Hendrati Pirc-Danoewinata; Metin Aytekin; Helm ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 203 KB

## Abstract In myeloid malignancies, chromosome rearrangements involving band 3q21 are associated with a particularly poor prognosis of the disease. Their sensitive and unequivocal detection is therefore of great clinical importance. In this report, we describe the establishment of an interphase fl

Sex chromosome markers: Characterization
Sex chromosome markers: Characterization using fluorescence in situ hybridization and review of the literature
โœ Schwartz, Stuart; Depinet, Theresa W.; Leana-Cox, Julie; Isada, Nelson B.; Karso ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 63 KB ๐Ÿ‘ 2 views

Fluorescence in situ hybridization (FISH) using biotin labeled X-and Y-chromosome DNA probes was utilized in the analysis of 23 sex chromosome-derived markers. Specimens were obtained through prenatal diagnosis, because of a presumptive diagnosis of Ullrich-Turner syndrome, mental retardation, and m