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Reutilization of previously hybridized slides for fluorescence in situ hybridization

✍ Scribed by Lucy Epstein; Sandy DeVries; Frederic M. Waldman

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 343 KB
Volume: 21
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990210410

No coin nor oath required. For personal study only.

✦ Synopsis

Application of fluorescence in situ hybridization (FISH) to clinical material is sometimes limited by sample size. In addition, heterogeneity among slides prepared from a single sample may lead to variation in FISH analyses. Reutilization of material for repeated FISH analyses would help to alleviate these problems. We have developed a simple procedure for repeated FISH analyses with directly conjugated probes. Previously hybridized probes are removed by incubation in denaturing solution, and slides can then be rehybridized without residual

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