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Sex chromosome markers: Characterization using fluorescence in situ hybridization and review of the literature

✍ Scribed by Schwartz, Stuart; Depinet, Theresa W.; Leana-Cox, Julie; Isada, Nelson B.; Karson, Evelyn M.; Park, Vicki M.; Pasztor, Linda M.; Sheppard, Linda C.; Stallard, Richard; Wolff, Daynna J.; Zinn, Arthur B.; Zurcher, Vickie L.; Zackowski, Joleen L.

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 63 KB
Volume: 71
Category: Article
ISSN: 0148-7299
DOI: 10.1002/(sici)1096-8628(19970711)71:1<1::aid-ajmg1>3.0.co;2-1

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✦ Synopsis

Fluorescence in situ hybridization (FISH) using biotin labeled X-and Y-chromosome DNA probes was utilized in the analysis of 23 sex chromosome-derived markers. Specimens were obtained through prenatal diagnosis, because of a presumptive diagnosis of Ullrich-Turner syndrome, mental retardation, and minor anomalies or ambiguous genitalia; three were spontaneous abortuses. Twelve markers were derived from the X chromosome and eleven from the Y chromosome; this demonstrates successfully the value and necessity of FISH utilizing DNA probes in the identification of sex chromosome markers. Both fresh and older slides, some of which had been previously G-banded, were used in these determinations. We have also reviewed the literature on sex chromosome markers identified using FISH.

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