Substituent effects on the binding of ethidium and its derivatives to natural DNA
β Scribed by W. Leupin; J. Feigon; W.A. Denny; D.R. Kearns
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 492 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0301-4622
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## Abstract Calorimetric titrations have been performed on the binding of ethidium and propidium to calf thymus DNA at temperatures in the 15β60Β°C range. Enthalpy changes (Ξ__H__~B~) derived from these experiments performed with the new Omega reaction calorimeter have a precision of Β±0.10 kcal/mol
## Abstract Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with singleβstranded DNA was detected by hydroxyapatite column chromatography. Five of the o