Ethidium bromide in a concentration range of 5.7 nM to 0.7 mM was determined amperometrically with an electrochemically activated glassy carbon electrode. From the relationship derived for the intercalation of EB to DNA and the equilibrated concentration of free ethidium bromide determined, the bind
Spectroscopic probe of the competitive binding of ethidium bromide and neomycin to DNA
β Scribed by Medini Kanta Pal; Jimut Kanti Ghosh
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 501 KB
- Volume
- 51
- Category
- Article
- ISSN
- 1386-1425
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The interaction of two natural protoberberine plant alkaloids berberine and palmatine with tRNA phe was studied using various biophysical techniques and molecular modeling and the data were compared with the binding of the classical DNA intercalator, ethidium. Circular dichroic studies revealed that
## Abstract Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with singleβstranded DNA was detected by hydroxyapatite column chromatography. Five of the o