Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays
โ Scribed by YuYing Liu; PengYe Wang; ShuoXing Dou; Ping Xie; XuGuang Xi
- Book ID
- 107365330
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 672 KB
- Volume
- 52
- Category
- Article
- ISSN
- 1001-6538
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๐ SIMILAR VOLUMES
## Abstract Changes in the conformation of poly(G), poly(C), poly(U), and poly(I) in the presence of divalent metal ions Mg^2+^, Ca^2+^, Mn^2+^, Co^2+^, Ni^2+^, Cu^2+^, Cd^2+^, and Zn^2+^ have been measured by means of ORD and u.v. spectra. Mg^2+^ and Ca^2+^ ions stabilize helical structures of all
The melting temperature of a natural DNA is decreased in the presence of increasing amounts of copper ions, whereas other divalent metal ions stabilize the DNA secondary structure a t low ionic strength. At 1.28 X lO-4M, Cuz+ produces a decrease of T,,, depending on base composition. At very low Cu\