Study of the relationship between estimates of enzyme kinetic parameters
β Scribed by K. Wang; R.I. Kitney; J.W. Seakins; M. Hjelm
- Book ID
- 108359995
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 523 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0022-5193
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Expressions are derived for the parameters that can be obtained from (1) steady-state kinetics, (2) isotope-exchange kinetics at equilibrium, and (3) equilibrium binding experiments for the following two one-substrateone-product enzymic mechanisms: It is shown that the exchange constants for both m
The efficiencies of estimates obtained from the direct linear plot (A. Cornish-Bowden and R. Eisenthal, 1978, Biochem. Biophys. Acta, 523, 268) are shown to be dependent on the spacing of substrate concentrations. When substrate values are harmonically spaced, the direct linear plot should not be us