A special mixing device for initiating enzyme-catalyzed reactions is used to rapidly achieve an unperturbed quasi-steady state. An on-line computer is employed to sample the initial conditions, the mixing time, and concentrations that change as a function of time during this quasi-steady state phase
โฆ LIBER โฆ
The relationship between steady-state kinetic parameters and equilibrium parameters for one substrate/one product enzymic reactions
โ Scribed by I.G. Darvey
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 436 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0022-5193
No coin nor oath required. For personal study only.
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A frequently used measure for the extent of cooperativity in ligand binding by allosteric proteins is the Hill coefficient. Hill coefficients can be measured for steady-state kinetic data and also for transient kinetic data. Here, the relationship between the two types of Hill coefficients is analys