Statistical evaluation of the direct linear plot method for estimation of enzyme kinetic parameters

✍ Scribed by R.C. Kohberger

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 403 KB
• Volume: 101
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90031-7

✦ Synopsis

The efficiencies of estimates obtained from the direct linear plot (A. Cornish-Bowden and R. Eisenthal, 1978, Biochem. Biophys. Acta, 523, 268) are shown to be dependent on the spacing of substrate concentrations. When substrate values are harmonically spaced, the direct linear plot should not be used. The nonparametric confidence limits based on the direct linear plot are accurate in their confidence coefficient, but their efficiencies are shown to be dependent on substrate spacing. Harmonic spacing is, in general, a more efficient experimental design for estimating K, than arithmetic spacing when the appropriate estimation methods are used. if assumptions about the error structure cannot be made, the best procedure for estimating K, is to have harmonic spacing of substrate values and use weighted least squares for estimation. The most accurate and precise estimation of enzyme kinetic parameters requires knowledge of the error structure and utilization of the appropriate nonlinear regression.