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Study of the fluorescence system deoxyguanylic acid-Tb (III) and the determination of deoxyguanylic acid

✍ Scribed by Jinghe Yang; Zuquan Gao; Nianqin Jie; Huabin Zou; Rongjiang Han; Weidong Zhao; Xianjuan Huang

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
324 KB
Volume
52
Category
Article
ISSN
1386-1425

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✦ Synopsis

It was found that deoxyguanylic acid (dGMP) can form a complex with Tb(|II) at pH 6.9 7.5, which then emits the strong characteristic fuorescence of Tb(IIl). Excitation is at 290 nm and emission wavelengths are 490 nm and 545 nm. A linear relation is obtained between the fluorescence intensity and dGMP concentration in the range 2.0x 10 7 to 1.0x 10 5 tool 1 ~. The detection limit is 3.0x 10 ~ mol I ~. This method can determine dGMP selectively in the presence of other nucleotides (not including GMP).

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