An automated assay for the estimation of S-aminolevulinic acid dehydratase activity in human erythrocytes has been devised. The present design allows the performance of thirteen analyses per hour. Samples are hemolysed by saponin in the flow system. Lateral interaction between consecutive samples is
Study of erythrocyte δ-aminolevulinic acid dehydratase activity in porphyria cutanea tarda
✍ Scribed by N. Simon; Mária Kiss
- Book ID
- 104891091
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 320 KB
- Volume
- 266
- Category
- Article
- ISSN
- 0340-3696
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A method preserving the activity of the erythrocytic enzyme 6-aminolevulinic acid dehydratase (EC 4.2.1.24) was developed using a vehicle of 50% aqueous glycerol containing dithiothreitol (80 pM). Whole heparinized blood (0.5 mi) was added to 0.75 ml of this mixture in a cryovial tube (capacity 1.3
## Abstract The use of ratios of erythrocytic δ‐aminolevulinic acid dehydratase activity measured at specific pH values, with or without prior incubation of the buffered hemolysate, was investigated as a possible alternative to the measurement of enzyme activity at a single pH value as a biologic i