𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Automated determination of δ-aminolevulinic acid dehydratase activity in human erythrocytes

✍ Scribed by Peter Bodlaender; David D. Ulmer; Bert L. Vallee

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
642 KB
Volume
58
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

An automated assay for the estimation of S-aminolevulinic acid dehydratase activity in human erythrocytes has been devised. The present design allows the performance of thirteen analyses per hour. Samples are hemolysed by saponin in the flow system. Lateral interaction between consecutive samples is negligible and repeatability of analysis is excellent. The analytical values of the automated and manual procedures correlate strongly. In randomly selected, hospitalized patients the mean value of S-aminolevulinic acid dehydratase activity is significantly lower than that found in a normal population.

📜 SIMILAR VOLUMES

A comparison of the effects of inorganic
A comparison of the effects of inorganic and alkyllead compounds on human erythrocytic δ-aminolevulinic acid dehydratase (ALAD) activity in vitro
✍ C. B. Burns; I. R. Godwin 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 786 KB

A study comprising two experiments comparing the effects of the alkyllead compounds triethyllead acetate and tetraethyllead as well as an inorganic lead compound (Pb(NO&) on human erythrocytic 6-aminolevulinic acid dehydratase activity was undertaken. In Experiment I , four human blood samples were

Method for preserving erythrocytic δ-ami
Method for preserving erythrocytic δ-aminolevulinic acid dehydratase activity that facilitates population studies on lead intoxication
✍ C. B. Burns; K. S. Sriprakash; J. R. Powers; B. J. Currie 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 410 KB

A method preserving the activity of the erythrocytic enzyme 6-aminolevulinic acid dehydratase (EC 4.2.1.24) was developed using a vehicle of 50% aqueous glycerol containing dithiothreitol (80 pM). Whole heparinized blood (0.5 mi) was added to 0.75 ml of this mixture in a cryovial tube (capacity 1.3

Erythrocytic δ-aminolevulinate dehydrata
Erythrocytic δ-aminolevulinate dehydratase activity as a function of pH. A study of activity ratios as biologic indicators of lead intoxication
✍ D. C. Wigfield; J.-P. Farant 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 638 KB

## Abstract The use of ratios of erythrocytic δ‐aminolevulinic acid dehydratase activity measured at specific pH values, with or without prior incubation of the buffered hemolysate, was investigated as a possible alternative to the measurement of enzyme activity at a single pH value as a biologic i