Method for preserving erythrocytic δ-aminolevulinic acid dehydratase activity that facilitates population studies on lead intoxication
✍ Scribed by C. B. Burns; K. S. Sriprakash; J. R. Powers; B. J. Currie
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 410 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0260-437X
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✦ Synopsis
A method preserving the activity of the erythrocytic enzyme 6-aminolevulinic acid dehydratase (EC 4.2.1.24) was developed using a vehicle of 50% aqueous glycerol containing dithiothreitol (80 pM). Whole heparinized blood (0.5 mi) was added to 0.75 ml of this mixture in a cryovial tube (capacity 1.3 mi), mixed well and stored in a freezer at -20°C for 21 days. Statistical comparison of the enzyme activity when freshly assayed and after storage indicated excellent agreement for quantitation of the non-activated enzyme (intraclass correlation coefficient = 0.99) and the activated enzyme (intraclass correlation coefficient = 0.98). This storage method will facilitate future population studies on lead intoxication, particularly those in remote locations.