𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Studies on the surface of chick blastoderm cells. III. Calcium binding to ionogenic sites

✍ Scribed by Harriet L. Harris; Sara E. Zalik

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
651 KB
Volume
83
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The effect of increasing calcium concentrations on the electrophoretic mobility of cells obtained from early chick blastoderms at successive developmental stages was studied. Cells had zero mobilities at calcium concentrations in the range of 1 to 2 × 10^−2^ M CaCl~2~, and became positively charged when calcium concentrations between 2 and 5 × 10^−2^ M CaCl~2~ were used. Results using trypsin suggest that while some calcium binding sites disappear from the surface ionogenic layer, the majority of them are not removed by this enzyme. The calcium binding capacity did not vary appreciably in the stages studied. Charge reversal induced by calcium is reversible.

📜 SIMILAR VOLUMES

Studies on the surface of chick blastode
Studies on the surface of chick blastoderm cells. I. Electrophoretic mobility and pH-mobility relationships
✍ Sara E. Zalik; Esmond J. Sanders; Carol Tilley 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 English ⚖ 657 KB

The electrophoretic mobilities (E.P.M.) and pH-mobility relationships of chick blastodenn cells at successive developmental stages were determined. Mobilities in 0.145 M NaC1, pH 7.2, showed a wide spread and decreased from a mean value of 1.63 p/sec/V/cm in the prestreak blastoderm, to 1.49 in the

Flow microfluorometric studies of lectin
Flow microfluorometric studies of lectin binding to mammalian cells II. Estimation of the surface density of receptor sites by multiparameter analysis
✍ John A. Steinkamp; Paul M. Kraemer 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 534 KB

## Abstract A flow‐system multiparameter cell analyzer that simultaneously measures and processes fluorescence and cell volume signals from single cells was used to study the binding of fluorescein‐conjugated Concanavalin A (Con A–F) to the cell surface. Cells reacted with Con A–F were passed throu

Quantitative studies on the binding of s
Quantitative studies on the binding of syngeneic antibody to the surface antigens of akr-virus-induced rat lymphoma cells
✍ Charles W. Boone; Phyllis R. Brandchaft; Dianne N. Irving; Raymond Gilden 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 503 KB

## Abstract The binding of rat antibody to G cell surface antigen (GCSA) of syngeneic, AKR‐virus‐induced lymphoma cells was studied by performing certain titration procedures using mixed immune and normal sera labelled with different iodine radioisotopes, a technique which permits precise correctio

Fourier transform infrared spectroscopic
Fourier transform infrared spectroscopic study on the Ca2+-bound coordination structures of synthetic peptide analogues of the calcium-binding site III of troponin C
✍ Masayuki Nara; Hisayuki Morii; Fumiaki Yumoto; Hiroyuki Kagi; Masaru Tanokura 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 245 KB 👁 2 views

## Abstract The coordination structures of Ca^2+^ ion bound to synthetic peptide analogues of the calcium‐binding site III of rabbit skeletal muscle troponin C (TnC) were investigated by Fourier transform infrared (FTIR) spectroscopy. The region of the COO^−^ antisymmetric stretching vibration prov