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Studies on the surface of chick blastoderm cells. I. Electrophoretic mobility and pH-mobility relationships

โœ Scribed by Sara E. Zalik; Esmond J. Sanders; Carol Tilley

Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
657 KB
Volume
79
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

The electrophoretic mobilities (E.P.M.) and pH-mobility relationships of chick blastodenn cells at successive developmental stages were determined. Mobilities in 0.145 M NaC1, pH 7.2, showed a wide spread and decreased from a mean value of 1.63 p/sec/V/cm in the prestreak blastoderm, to 1.49 in the intermediate streak and 1.34 in the head process stages. Cell surface charge density changes as detected by E.P.M. were observed in all regions of the embryo.

pH-mobility relationship studies showed that two cell populations differing i n their approximate isoelectric point appeared at the intermediate streak and head process stages. Neuraminidase treatment indicated that the contribution of sialic acid residues to the negatively very small at the stages studied.

๐Ÿ“œ SIMILAR VOLUMES

Studies on the surface of chick blastode
Studies on the surface of chick blastoderm cells. III. Calcium binding to ionogenic sites
โœ Harriet L. Harris; Sara E. Zalik ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 651 KB

## Abstract The effect of increasing calcium concentrations on the electrophoretic mobility of cells obtained from early chick blastoderms at successive developmental stages was studied. Cells had zero mobilities at calcium concentrations in the range of 1 to 2 ร— 10^โˆ’2^ M CaCl~2~, and became positi