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Quantitative studies on the binding of syngeneic antibody to the surface antigens of akr-virus-induced rat lymphoma cells

✍ Scribed by Charles W. Boone; Phyllis R. Brandchaft; Dianne N. Irving; Raymond Gilden

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
503 KB
Volume
9
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The binding of rat antibody to G cell surface antigen (GCSA) of syngeneic, AKR‐virus‐induced lymphoma cells was studied by performing certain titration procedures using mixed immune and normal sera labelled with different iodine radioisotopes, a technique which permits precise correction for non‐specific adsorption. Using plots of titration data which conformed to linear equations derived from the mass action law, we determined the following constants: (1) The average density of antibody molecules bound to GCSA sites was 6.9 × 10^6^ per cell, and 11,300 per square micron; (2) The average equilibrium constant of the GCSA‐antibody association reaction was 6.54 × 10^6^ ℓmole^−1^; (3) The mean binding affinity (ΔF°) of the GCSA‐antibody bond was —9.17 Kcal mole^−1^; (4) The rate constant for antibody dissociation at 0°C was 3.67 × 10^−5^ sec^−1^.

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