## Abstract An approach has been developed for predicting the change of the aqueous phase pH due to the partition of amino acid substrates in aqueous–organic biphasic systems. In the model reaction of papain‐catalyzed synthesis of __N__‐benzyloxycarbonyl‐glycine‐L‐phenylalanine methyl ester (ZGPM)
Studies on enzymatic peptide synthesis in biphasic aqueous-organic systems with product extraction
✍ Scribed by Peter Kuhl; Regina Schaaf; Hans-Dieter Jakubke
- Book ID
- 104953796
- Publisher
- Springer Vienna
- Year
- 1987
- Tongue
- English
- Weight
- 448 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0026-9247
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N-(benzyloxycarbonyl)-L-aspartic acid (Z-L-Asp) has generally been used as a carboxyl substrate for the enzymatic synthesis of a precursor of aspartame (synthetic sweetener); however, alternative inexpensive protection groups have been in demand for lowering the total cost of its industrial-scale pr
The ability of Qnara cahnch L. protease to perform pepticle synthesis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZPhe and MetOMe. The reaction products were separated by BP-HF'LC and identified by amino acid analysis and by El-MS. Significant amounts o